Trita^® Human Identifier Kit

Catalog number

ID-1621.25

ID-1621.50

ID-1621.100

Application area

The Trita® Human Identifier kit is designed to determine the kinship relationships among individuals and determine human identity in the context of forensic medicine. It is also applied to research activities in the field of molecular biology. The underlying foundation of this kit lies in the Multiplex fluorescent PCR molecular method, which enables the simultaneous examination of 16 genetic markers in the genome (DNA) obtained from various human biological samples such as blood, hair, nails, and bones. It is important to note that this particular product is not suitable for diagnostic, preventive or therapeutic measures.

product performance principle

The Trita® Human Identifier Kit developed by TritaGene is designed to identify human identity in forensic medicine, establish kinship relationships, and determine the genetic patterns of individuals. This particular kit consists of 16 Short Tandem Repeat (STR) polymorphic genetic markers, which include all 13 STR markers approved by CODIS, one STR marker endorsed by the European Union (ENFSI), the SE-33 marker and the Amelogenin sex determination marker. These markers are detected and amplified through a multiplex fluorescent PCR reaction.

The utilization of polymorphic STR markers holds considerable significance in human identification and proves to be effective in genotyping minute quantities of DNA samples. The SE-33 marker boasts a remarkably high heterozygosity, the inclusion of which enhances the accuracy and reliability of the kit’s results. Furthermore, the implementation of a five-dye fluorescent system technology enables the efficient separation of genetic markers, thereby providing comprehensive outcomes in a brief timeframe. The identification process using this product involves the following procedural steps:

• DNA extraction from human biological samples (blood, hair, nails or other human biological samples)
• Amplification of desired gene markers by Multiplex fluorescent PCR method (using Trita® Human Identifier kit)
• Identification of amplicons by capillary electrophoresis method using genetic analyzer device
• Analyzing and interpreting the results using the GeneMarker® or GeneMapper® software

It is worth noting that the fluorescent colors employed in this particular product are shown in the subsequent table.

Main features of Trita® Human Identifier Kit

• Intended for human identification in forensics and DNA paternity testing.
• Simultaneous amplification of 13 original CODIS loci,1 marker from ENFSI, the SE33 locus and
• Based on a single multiplex fluorescent PCR amplification and Capillary electrophoresis.
• Efficient separation of the 15 STR loci and Amelogenin using a five-dye fluorescent system technology.
• Uses Extracted DNA from biological samples such as blood, hair, nail, etc.
• High discriminating ability due to SE33 locus analysis
• Highly sensitive to amplify and detect little concentration of temple DNA due to STR markers analysis.
• Result analysis using professional softwares such as GeneMapper® or GeneMarker®.
• Manufactured by TritaGene Biotech Co. within quality management systems accredited to ISO 9001:2015.

Composition of Trita® Human Identifier Kit

Trita® Human Identifier Kit is available in 25, 50 and 100 test models. The table below displays the kit’s components and their values in various models of this product.

Attention:

This kit contains an internal allelic ladder which is considered a qualitative standard indicator for evaluating the accuracy of genotyping STR markers. This ladder is integrated into the IDS-1 solution, serving as one of the constituents within the kit.

Technical specification of Trita® Human Identifier Kit