Catalog number
MSI-223.25
Application area
The Trita® MSI Analysis Kit has been designed to identify the instability of microsatellite repeat sequences in human cells. This is achieved by utilizing the Multiplex fluorescent PCR method along with specific primers that are labeled with four distinct fluorescent colors (6-FAMTM, VIC®, NEDTM, PET®). The kit focuses on investigating specific repetitive sequences associated with the MMR system. These sequences encompass five mononucleotide markers (BAT-25, BAT-26, NR-21, FGA, NR-24 & MONO-27) that aid in determining the MSI status in genomic DNA, as well as a pentanucleotide marker (Penta D) that ensures the control of reaction contamination.
To detect the PCR products, capillary electrophoresis is employed, utilizing a genetic analyzer [models 3130(XI) and 3500(XI)]. The ultimate results can be interpreted and analyzed through the utilization of the GeneMarker® software, which is based on the panel kit. The subsequent table illustrates the characteristics and specificities of the markers examined by this particular product.
| Locus information of Trita® MSI Analysis Kit | |||
| Dye | Size range
(bp) |
Major Repeat
Sequence |
Markers |
| Blue | 130-160 | (A)21 | NR-21 |
| Blue | 99-120 | (A)26 | BAT-26 |
| Blue | 80-99 | (A)25 | BAT-25 |
| Red | 115-140 | (A)24 | NR-24 |
| Green | 130-160 | (A)27 | MONO-27 |
| Yellow (Black) | 80-120 | (CTTT)12-51 | FGA |
| Yellow (Black) | 140-226 | (AAAAG)2–17 | Penta D |
Main features of Trita® MSI Analysis Kit
- Detection of microsatellite instability (MSI) in patients with colorectal cancer or other MSI-relevant cancers
- Evaluation of insertions or deletions in microsatellite markers (BAT-25, FGA, BAT-26, NR-21, NR-24 and MONO-27) and Penta D
- Based on multiplex Fluorescent PCR and Capillary Electrophoresis techniques
- Validated with DNA extracted from plasma and formalin-fixed paraffin-embedded (FFPE) tissues
- High sensitivity and specificity
- Manufactured according to ISO 13485:2016 and ISO 9001:2015
Composition of Trita® MSI Analysis Kit
Trita® MSI Analysis Kit is available in 25, 50, and 100 test models. The table below displays the kit’s components:
| Composition of Trita® MSI Analysis Kit | ||||
| Tube/Cap color | Vol. for 100 rxn (μl) | Vol. for 50 rxn (μl) | Vol. for 25 rxn (μl) | Kit Contents |
| Amber/Yellow | 4×125 | 2×125 | 125 | MSI-1 |
| Clear/Red | 4×375 | 2×375 | 375 | MSI-2 |
| * The kit contains a Quick Manual inside the package. | ||||
Technical specification of Trita® MSI Analysis Kit
The following table summarizes the most important technical features of the Trita® MSI Analysis Kit product:
| Technical specification of Trita® MSI Analysis Kit | |
| Kit Specifications and characteristics | Parameters |
| Multiple Fluorescent Probe Amplification (MFPA) | Technique |
| Semi-quantitative | Type of Analysis |
| Mononucleotide markers (BAT-25, FGA, BAT-26, NR-21, NR-24 & MONO-27)
Pentanucleotide marker (Penta D) |
Target Sequence |
| Formalin-Fixed Paraffin-Embedded (FFPE) human tissues | Validated Specimen |
| -20 ± 5 °C | Storage |
| 6-FAMTM, VIC®, NEDTM, PET® | Dye |
| 50-80 ng/rxn | Validated DNA Conc. |
| Maxwell® 16 FFPE Tissue LEV DNA Purification Kit (Promega-USA)
ReliaPrep™ FFPE gDNA Miniprep System (Promega-USA) QIAamp® DNA FFPE Tissue Kit (Qiagen, Germany) MagneSil® Genomic, Fixed Tissue System (Promega-USA) |
Validated Extraction Method |
| BioRad (T 100), Applied Biosystem (Veriti), GeneAmp PCR System 9700 (Ramping rates: heating 0.8 ̊C/s, cooling 1.6 ̊C/s) |
Validated thermocyclers |
| Applied Biosystems™ 3130/3130 xL Genetic Analyzer Applied Biosystems™ 3500/3500 xL Genetic Analyzer |
Validated Genetic analyzers |
| 560 SIZER ORANGE (Devyser-Sweden) GeneScan™ 500 LIZ® Size Standard (Life Technologies-USA) GeneScan™ 600 LIZ® Size Standard (Life Technologies-USA) |
Size standard |
| GeneMarker® | Validated Softwares |
