Trita^® α-thalassemia InDel Detection Kit

Catalog number

TA-822.25

TA-822.50

TA-822.100

Application area

The Trita®α-thalassemia InDel Diagnostic Kit has been developed and manufactured to identify deletions of -α^3.7, -α^4.2, -α^20.5, —^MED and the occurrence of α-triplication within the cluster of the Human alpha globin gene situated on the short arm of chromosome 16 which leads to alpha thalassemia disease. The product operates on the basis of a novel technique known as Multiple Fluorescent Probe Amplification (MFPA). By extracting DNA from peripheral blood of both infants and adults, this diagnostic kit can accurately diagnose alpha-thalassemia.

product performance principle

The Trita®α-thalassemia InDel Diagnostic Kit examines 15 markers and has probes and primers labeled with 6-FAM^TM fluorescent dye. the human alpha-globin gene is specifically achieved through the utilization of fluorescent probes and primers in a multiplex reaction. Subsequently, the resulting product of the MFPA reaction can be accurately identified by subjecting it to capillary electrophoresis using a genetic analyzer. The monochromaticity of the product does not require the calibration matrix of the genetic analyzer. GeneMarker® and T-Lyser® softwares are utilized to analyze and interpret the final results, which were originally created and introduced by TritaGene. This product’s ability to detect alpha-thalassemia disease is based on a three-stage process that is thoroughly validated according to the following criteria.

DNA extraction from biological sample

Validation of this product has been done using human peripheral blood samples obtained from either adult individuals or infants. Furthermore, this product has been specifically designed to enhance efficiency when utilized in conjunction with column-based DNA extraction kits, such as the QIAamp® DSP DNA Blood Mini Kit, manufactured by Qiagen, Germany. Sedimentary DNA extraction methods are also useful.

The Molecular reaction known as Multiple Fluorescent Probe Amplification (MFPA) consists of a series of steps:

• Denaturation
• Probe Hybridization
• Amplification of desired gene markers using the PCR method (PCR Amplification)

Fragment Analysis comprises of several steps:

• • Fragment Separation by Capillary Electrophoresis
  • Data analysis and interpretation of the results were performed using GeneMarker® software and then with the new T-Lyser® software.

Main features of Trita®α-thalassemia InDel Diagnostic Kit

• Detection of large genetic deletions including -α⁷، -α ^4.2، -α ^20.5، —^MED and α-triplication in alpha-globin gene.
• Based on Multiple Fluorescent Probe Amplification (MFPA®) and Fragment analysis.
• Double checking the occurrence of InDels through amplification of two individual markers
• A fast and easy method with high sensitivity and specificity.
• DNA-to-Data in less than four hours through a single PCR reaction and capillary electrophoresis.
• Manufactured within the quality management systems (ISO 13485:2016 and ISO 9001:2015).

Composition of Trita®α-thalassemia InDel Diagnostic Kit

Technical specification of Trita®α-thalassemia InDel Diagnostic Kit

In the table below, the most important technical features of the Trita® α-thalassemia InDel Diagnostic Kit product are presented in brief: